biological systems using lipidomics
The mass spectrometry (MS) resources on campus aim to provide researchers with state-of-the-art techniques and equipment to characterize biomolecules including proteins and peptides (proteomics, sugars including glycoproteins (glycemic), metabolites (metabolomics) and lipids (lipidomics). A number of state-of-the-art instruments for the separation of biomolecules and their analysis by mass spectrometry-based approaches are available. In addition to providing analytical services, the mass spectrometry facility on campus provides training to on-campus scientific staff on the use of this technology as well as develop new analytical methods required to facilitate on campus research.
Facility is driven to establish a hyphenated Mass Spectrometry based Lipidomic platform in basic biology, clinical studies and biotechnology at Bangalore Life Sciences Cluster (Bliss) affiliated institutes; NCBS, CCAMP and In Stem. The platform undertakes clinical, biological, chemical, ecology studies of lipidomic/ metabolites for Bliss community and external researchers. Direct infusion as well as LC-MS/MS methods using High Resolution Mass Spectrometry (HRMS) like Orbitrap technology (Q-Enactive, Orbitrap XL) are available.
Identification and quantification of known and unknown Lipids/metabolites in biological fluids such as plasma, urine, saliva and tissue using Data dependent (DDA) and data independent (DIA) analysis for the estimation of lipids and other metabolites. Comprehensive lipid profiling and quantitation is a system-based study of all possible lipid classes in the biological system. Phosphoinositide’s measurements in cell signaling.
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Journal of Chromatography & Separation Techniques